| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054859 |
| Chromosome: | chromosome 9 |
| Location: | 5972833 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412300 | PAM16,TIM16 | Presequence translocase-associated protein import motor subunit; (1 of 1) K17805 - mitochondrial import inner membrane translocase subunit TIM16 (PAM16, TIM16) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACTGCGCCTATCCTCCGCCATGCCTCCCCACCTCAGCCCCTCCCCTCT |
| Internal bar code: | TGCGTAAAATCGAGTGGAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1033 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAAGGCGAAGTATGGGGG |
| Suggested primer 2: | CATCCATCCTCTCGCCAGAC |