| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.054863 |
| Chromosome: | chromosome 6 |
| Location: | 1117204 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257700 | RAD18 | Putative E3 ubiquitin ligase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGCCGTTGCTGCTGCAGGTCAGACAGGCGAGCCAGGTCCTGCTGCA |
| Internal bar code: | TCGTATATTATATGTATTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3235 |
| LEAP-Seq percent confirming: | 29.4118 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGTGGTCTTGGAGAAGC |
| Suggested primer 2: | GCGCTCTCCATCTCTGTCTC |