| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054874 |
| Chromosome: | chromosome 3 |
| Location: | 4182257 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173350 | ANK22b,ANK22 | (1 of 1) PTHR24193//PTHR24193:SF88 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED; Predicted protein with ankyrin repeats | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATAATCATCTAGCAAAAATGTTCCCTGGAATGCCTGCGGGCCCCGGT |
| Internal bar code: | TATGGAGAGTAATCGCTTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1561 |
| LEAP-Seq percent confirming: | 45.8333 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGACTTGCCCAGCTTCTC |
| Suggested primer 2: | ATGCTTTAAGCGAGGCCCTT |