| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.054883 |
| Chromosome: | chromosome 10 |
| Location: | 280166 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419600 | (1 of 2) PF07823 - Cyclic phosphodiesterase-like protein (CPDase) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCCACTGCTCTTTGGCGCGACTTGGCGAGCGCGGGCAAGCGCTGGCT |
| Internal bar code: | GGTGACCCACGTCAGTGAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5810 |
| LEAP-Seq percent confirming: | 89.2308 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGACATCCTCTAGCCA |
| Suggested primer 2: | AAGCACGTCAAGGTAGGTGG |