| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054903 |
| Chromosome: | chromosome 6 |
| Location: | 831322 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255500 | (1 of 1) PTHR10992:SF743 - ALPHA/BETA-HYDROLASE DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTGGGGCGGCGCTCGTCGGCCCCGGCGCGTGTTGCTCTCCGCATGCG |
| Internal bar code: | GGGGTTAAAGAAGTTCAGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1442 |
| LEAP-Seq percent confirming: | 97.3684 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACATAGGATGCCGGGAC |
| Suggested primer 2: | CGTAGATGGCAGACCTCCAC |