Insertion junction: CLIP2.054907_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.054907
Chromosome:chromosome 11
Location:1450748
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g469500 FXL6 FixL-like PAS domain protein; (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGACGCACAGGTGCAGCACCGCCACAAACAACGCCACAGACTGCAAAGT
Internal bar code:GCTAAACGTGAGGCTACTCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2971
LEAP-Seq percent confirming:47.5
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:ATAGCCTTGCCAGCAACACT
Suggested primer 2:GGATGCGCACCATTGACATC