Insertion junction: CLIP2.054910_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.054910
Chromosome:chromosome 2
Location:5716336
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre02.g116400 FAP120,DII7 Flagellar Associated Protein 120; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACACGCCCTCGCTCCTCCCTGCTACCCGCTCCCCACCAGTCCGG
Internal bar code:GACCTATAACGGGTTAATAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:113
LEAP-Seq percent confirming:1.88679
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:52
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCCGCTTGCGCTATCTA
Suggested primer 2:GACGGGGAAGAGCAACTTGA