| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.054915 |
| Chromosome: | chromosome 9 |
| Location: | 5188964 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406700 | (1 of 1) PTHR10795//PTHR10795:SF371 - PROPROTEIN CONVERTASE SUBTILISIN/KEXIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAAGCCGGCATCGCGTGGATCGCAGCGCGTGACATCGTGATCTGGAT |
| Internal bar code: | GTAGTTGATGAATGTTAAACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1279 |
| LEAP-Seq percent confirming: | 44.4444 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGCGTGGTGTTTGCAC |
| Suggested primer 2: | CAGCTCGTACCGGATCTGTC |