| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.054923 |
| Chromosome: | chromosome 1 |
| Location: | 5225422 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036600 | EXN3 | (1 of 2) K14570 - RNA exonuclease 1 (REX1, REXO1, RNH70); Exoribonuclease II | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCTTCACTGGGTCCGCCTCCCCAGAGGCCGACTTCGCCGCCTTGCGC |
| Internal bar code: | TATTGTCGATATTGGTGAATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1431 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGCCTTTGTCTGGGAGT |
| Suggested primer 2: | CGCTGCCTACATGTACCGAT |