| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054965 |
| Chromosome: | chromosome 6 |
| Location: | 6348157 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293100 | (1 of 2) PTHR19957:SF124 - SYNTAXIN-8B-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCAACAACGCGGGGCTGCTGGGCCCCGACACGGGTTTCACACGCGCG |
| Internal bar code: | GCGCGTGGACGACTCCCGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3153 |
| LEAP-Seq percent confirming: | 83.5294 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACATCGACGAGCAGGTAG |
| Suggested primer 2: | TACGGCTCTGGGGAGATGAA |