| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.054977 |
| Chromosome: | chromosome 17 |
| Location: | 692242 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700650 | GFY2 | (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAGTAGTCAAACATCAAGAAGCAGCAGCCGTGAGGACTTCACGATGTC |
| Internal bar code: | TTTCGTCGGATGTGATATACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1288 |
| LEAP-Seq percent confirming: | 30.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAACCCGCCCTATACCCA |
| Suggested primer 2: | CTAGGATGCTCAGACGCAGG |