Insertion junction: CLIP2.054982_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.054982
Chromosome:plastome
Location:23721
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802274 2717055,ChreCp012,rpl2 50S ribosomal protein L2; (1 of 2) IPR005880 - Ribosomal protein L2, bacterial/organellar-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAGGAATCTAATCCCCATAATTATGTTTTTAAGTTGTTTTACTATTTA
Internal bar code:CGTTTGTGTTACCTTTGATCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:57
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGCCAATGCGTAGTTGA
Suggested primer 2:CATGGTGTAACTGGACGGCT