Insertion junction: CLIP2.054995_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.054995
Chromosome:chromosome 3
Location:1236367
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g149650 CCD1 related to carotenoid 9%252C10-9'%252C10' cleavage dioxygenase; (1 of 1) K11159 - carotenoid cleavage dioxygenase (K11159) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTTGGGGTGGCCGGGCTGCGGTGACGGCCCTCGCACCTGCGTCACTCC
Internal bar code:AGGGTTAGAACAGCGTAAAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:226
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCCATCGACCATAAACGC
Suggested primer 2:GCAGCTTACTATGCGCACAC