Insertion junction: CLIP2.055025_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.055025
Chromosome:chromosome 7
Location:2205553
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g327200 (1 of 3) PF00534//PF13579 - Glycosyl transferases group 1 (Glycos_transf_1) // Glycosyl transferase 4-like domain (Glyco_trans_4_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAACGCACCCAGACATGCGGGAACGCAAAGGCGTGACCTCACCTTGGG
Internal bar code:TTTCTAACGTTCGTAACTCCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2151
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACGCGGACTATCTGGTC
Suggested primer 2:GTTGTCCCTCTACCACACGG