| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055039 |
| Chromosome: | chromosome 3 |
| Location: | 3180312 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165050 | PMA4 | (1 of 3) K01535 - H+-transporting ATPase (E3.6.3.6); Plasma membrane P-type calcium ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCCTGTACGCACTGTGTTGTATGGAGCTATGCAAACACCACCATACC |
| Internal bar code: | CGTGTTCGTCTATGACGTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 82.9268 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATACCCCGTTGAAGACC |
| Suggested primer 2: | TGCATGTGGCTTATCCGTGT |