Insertion junction: CLIP2.055045_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.055045
Chromosome:chromosome 1
Location:1422791
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g007550 GT90-1,GT90F1 GT90 family protein 1; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGTTTTCCTCTCAGTGCCCTGCTTGAATAATACAACCCAAACGACACA
Internal bar code:TTTCTACAATGATGAGACCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:691
LEAP-Seq percent confirming:44.4444
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCCGCCTCTGCTGATAAT
Suggested primer 2:ACACACATGCACCTGTCACA