| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.055046 |
| Chromosome: | chromosome 2 |
| Location: | 6885196 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388726 | (1 of 1) 3.1.3.32 - Polynucleotide 3'-phosphatase / 2'(3')-polynucleotidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCACCTGACTGCTAGCTCCCGCCCTGTGCCCTGATCTTCAACTAACCA |
| Internal bar code: | ATCGTATGAATAAGAGGCGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3475 |
| LEAP-Seq percent confirming: | 94.3396 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTGCTAGTTGCCCTAA |
| Suggested primer 2: | TCCATGATGGAGTCGATGCG |