| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055050 |
| Chromosome: | chromosome 12 |
| Location: | 3273749 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499300 | PDE31 | (1 of 4) K13293 - cAMP-specific phosphodiesterase 4 (PDE4); 3'%252C5'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTCAACCAGCAGCCGCACAATACGCGCCATCAGCCTTGCTGAACGGG |
| Internal bar code: | ACTCAACCGCAACCTAGGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4774 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAACAAGAGGCACTGGC |
| Suggested primer 2: | CAACAGGTGGCTGCAAAGTC |