Insertion junction: CLIP2.055055_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.055055
Chromosome:chromosome 8
Location:3728100
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g380550 SDR14 (1 of 2) IPR002347//IPR013968 - Glucose/ribitol dehydrogenase // Polyketide synthase, ketoreductase domain; Short-chain dehydrogenase/reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGCTCAGCCGCGTGGCCCGTGTGGCGCGCAACGGGGTTTGGCGCTGT
Internal bar code:TAATAAATCGGATATAACGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:176
LEAP-Seq percent confirming:2.98507
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:65
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTCCCACTCTCCAATTCC
Suggested primer 2:CGCAATCTTCACCTGCATCG