| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055057 |
| Chromosome: | chromosome 7 |
| Location: | 3772680 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338400 | (1 of 1) K14845 - RAT1-interacting protein (RAI1, DOM3Z) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTTGTGCGTCAGTGCCACGCGTCAGGACACCGGGGCTGAGGTGGAGG |
| Internal bar code: | ATGCAATAAAGCGGGGACGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1082 |
| LEAP-Seq percent confirming: | 89.7959 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAGAGTAGACGAGGCGGC |
| Suggested primer 2: | AAGCACCAGGTCCAACCAAA |