Insertion junction: CLIP2.055057_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.055057
Chromosome:chromosome 10
Location:5864400
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACGCACGCTGGCGCTGGTGTTAGTTGGTAGGCGGGGTAGGGAGGCACGC
Internal bar code:GCCCGCTGGGGAAACTATACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:745
LEAP-Seq percent confirming:25.8621
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTGCGCAGATTTAGCCTC
Suggested primer 2:TCAGTCGTCTAGGTCCAGCA