| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055059 |
| Chromosome: | chromosome 9 |
| Location: | 5480995 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408851 | (1 of 2) 3.4.11.9 - Xaa-Pro aminopeptidase / X-Pro aminopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCGGACGAAGTCGTTACCACATTTGCGGCCGTCCGGGCGGCCGCGCT |
| Internal bar code: | TAGTGACCGGCGCCAGGCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 21.5385 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGAGCGTGTGTAAGGAA |
| Suggested primer 2: | CGTCTGGGCCTGTTTTGTTG |