| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055060 |
| Chromosome: | chromosome 3 |
| Location: | 1342794 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150650 | (1 of 2) PTHR12775:SF0 - PROTEIN RTF2 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGACCCACCCGTGGGGTTCACAGGGATGCTCTCGAGCTCCGCCAGCTT |
| Internal bar code: | GTCTGGGACCGTGCGGTCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2088 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGTCAACCCGGAAGAGG |
| Suggested primer 2: | GCAGCAGAACGTCTCCTTCT |