| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.055065 |
| Chromosome: | chromosome 9 |
| Location: | 3473972 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396102 | (1 of 1) K08856 - serine/threonine kinase 16 [EC:2.7.11.1] (STK16) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCCTTCACACGCGGTCACGCGCCCCACTCCCCCCCTCCCCACCTGCT |
| Internal bar code: | CCTCTGATTTGCGAAGGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 271 |
| LEAP-Seq percent confirming: | 29.4118 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCAGGTCGCAAAACGCAG |
| Suggested primer 2: | TAGTCCAGCAGGGGCAGTAT |