Insertion junction: CLIP2.055066_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.055066
Chromosome:chromosome 12
Location:7220053
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g559053 CSB50 {"Probable transposon-derived protein of Chlamydomonas-Specific family B; (1 of 20) IPR010095//IPR013083 - Transposase IS605, OrfB, C-terminal // Zinc finger, RING/FYVE/PHD-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAATTGCTGGTGGGTGGGTGGGCCGCTGGGTTTATGCCTGGCTTTAGC
Internal bar code:TAGTTCCCCTGTAATGAGTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1903
LEAP-Seq percent confirming:18.1818
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGCCCCGACATTTTGGAC
Suggested primer 2:TTATGGAAACCGGGTCAGCC