Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.055085 |
Chromosome: | chromosome 13 |
Location: | 4870165 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g801581 | (1 of 17) 3.1.4.53 - 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGCACCCCGTCAGGTTGCCAGCTCCGCAACAGCTGCCCCGGCACCAA |
Internal bar code: | GAGTCAAAATCGACGTACAGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 811 |
LEAP-Seq percent confirming: | 51.8519 |
LEAP-Seq n confirming: | 14 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGATGTGGATGTGCGGATA |
Suggested primer 2: | AGACCTCGACGACAGGATGA |