| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055109 |
| Chromosome: | chromosome 16 |
| Location: | 3644570 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675602 | (1 of 1) K12669 - oligosaccharyltransferase complex subunit gamma (OST3, OST6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTGTTTCCCTATTGCCGCCTGCAGGTTCAAGCTGTGGAACTTGAAGAC |
| Internal bar code: | AGCTATTGTGGGAGAGAAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1257 |
| LEAP-Seq percent confirming: | 41.7722 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCGCGGTGTCTATGGCAC |
| Suggested primer 2: | GACTGGCCGCTCATACTACC |