Insertion junction: CLIP2.055122_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.055122
Chromosome:chromosome 13
Location:1202468
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g569900 (1 of 100) IPR001810 - F-box domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCAATGGGAGCGTGAGGGGTCAGGCGCGCTATTCGCACGCATGCATAG
Internal bar code:GGTGGGGAGAAGATAGGTCCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2350
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:TCCACTCAACAAGTCTCGCC
Suggested primer 2:GCCTTCCCACATGTCTGTCA