| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.055131 |
| Chromosome: | chromosome 10 |
| Location: | 3725593 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g801156 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGGTCCGGTGACGGCGCCCTCAGTGATGCCGAGCTGATGGCAGCGCT |
| Internal bar code: | TTCTTCACATGCGGAACTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2765 |
| LEAP-Seq percent confirming: | 5.55556 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGATCAGGCCTTGGAGGTA |
| Suggested primer 2: | GTCCTGCAACTGGAGAGCTT |