| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055135 |
| Chromosome: | chromosome 11 |
| Location: | 3848068 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACACACATATACACACACACACACCGAGTGCCTCCGCAACGGG |
| Internal bar code: | GGAATTACTCTTGGCGTCTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 160 |
| LEAP-Seq percent confirming: | 30.9091 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGGGGCCAGGAGCTTAT |
| Suggested primer 2: | GTGTCTCATGTGTGCCCTCA |