Insertion junction: CLIP2.055138_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.055138
Chromosome:chromosome 2
Location:3055920
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095750 ZEP1,ZXE1 (1 of 1) 1.14.13.90 - Zeaxanthin epoxidase / Zea-epoxidase; Putative zeaxanthin epoxidase-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCTCCTCCCACCCGCACACGCACCTCGAACGCCGACCGCGCGCCCTG
Internal bar code:CCTCGAGCGCCGGCTATATTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:649
LEAP-Seq percent confirming:30.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATAGTGCACTGTGCTGGCTT
Suggested primer 2:GTGAAGGCGAGATACGGGAG