Insertion junction: CLIP2.055145_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.055145
Chromosome:chromosome 8
Location:1532659
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g365000 CSE1 Protein of unknown function; (1 of 3) PF07887 - Calmodulin binding protein-like (Calmodulin_bind) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTACGCTCGTTCGCCACGCGCTTTGCTTCACAGCATGCAGCACACCTGT
Internal bar code:TAGAAATTTAGAGACCTTGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1157
LEAP-Seq percent confirming:47.3684
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CCGACCCTCTTCTTTGTGCT
Suggested primer 2:GGTTGACCGGAGTGTTTTGC