Insertion junction: CLIP2.055164_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.055164
Chromosome:chromosome 8
Location:694104
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g360500 ERM2 (1 of 1) PF13967//PF14703 - Late exocytosis, associated with Golgi transport (RSN1_TM) // Cytosolic domain of 10TM putative phosphate transporter (PHM7_cyt); ERD4-related membrane protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTGGTGAGAGCCGCGCAACAACGCCCCCGGCTGGGCTCTTAGGGGAGG
Internal bar code:TGCATGCAGGATTTATTGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:748
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCAACAGTCGTGACGTTA
Suggested primer 2:TACATGCGCATCCTGTGGTT