| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.055170 |
| Chromosome: | chromosome 16 |
| Location: | 2048277 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657000 | (1 of 1) K03134 - transcription initiation factor TFIID subunit 10 (TAF10) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTACTCCGGGCCGCTCCACCCGGCGGCCCCGGACCTCACTGAGCGACT |
| Internal bar code: | TATTTTTGCAAACCACAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3057 |
| LEAP-Seq percent confirming: | 92.4528 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTTGTGAGTGTGTGCAGG |
| Suggested primer 2: | GCGTTATGAAGCGTGCAGAG |