| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055172 |
| Chromosome: | chromosome 8 |
| Location: | 1137392 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCAGGCGAACTCCGGGTCCGTTTCCGGCAGCCCCGCCAGCTTTTTGA |
| Internal bar code: | AATAAAGGGGGGGTCGGCGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2634 |
| LEAP-Seq percent confirming: | 48.3871 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACAAGGCCCACGTCAAG |
| Suggested primer 2: | GATGACGGCTCTGACCTAGC |