Insertion junction: CLIP2.055173_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.055173
Chromosome:chromosome 10
Location:5871054
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g460600 CYN53 (1 of 2) PTHR11071//PTHR11071:SF297 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 53 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATGGTTTGGCGCTTCCCGTCGCCCGCCGTGGGCCGATCCCTGGTTTG
Internal bar code:GGAATTGTCGGCGTGCATTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1277
LEAP-Seq percent confirming:13.1579
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGAGGAGTACGGTATGTG
Suggested primer 2:TTTTGCTGGTGGGAATTGCG