Insertion junction: CLIP2.055182_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.055182
Chromosome:chromosome 2
Location:6722391
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g387467 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTTGCATATGCCTTCCGCAGTCACGTCATGGACCAAGTACAGCACGGA
Internal bar code:CGATGACATGATATGTCGTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2188
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTTGTACTGCGTGGTGTA
Suggested primer 2:ACTGCTTTTTCCGCTGCTTG