| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055201 |
| Chromosome: | chromosome 7 |
| Location: | 4439273 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343300 | (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCATGTTCCCCGGCAGGAACATGAGCGTGTGGGAGAATGCGCTTGCTG |
| Internal bar code: | CTCACTGTCAAGCGCTCTCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2996 |
| LEAP-Seq percent confirming: | 63.8889 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATACGAGTAGCGAGCCT |
| Suggested primer 2: | ACTTCATGCCTTCCCGTCAG |