Insertion junction: CLIP2.055216_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.055216
Chromosome:chromosome 4
Location:2765117
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g223650 FAP30 (1 of 1) PF02494 - HYR domain (HYR); Flagellar Associated Protein 30 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTGGTGCCGTGACGCCCGCATGCAACCATGCCTCTGGTGACTCTTTCC
Internal bar code:TTCAGGTAACGCATCGAAAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1214
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACGTCTGGAGCTGTGTT
Suggested primer 2:GATCGCCGCAATCAGTTGAC