Insertion junction: CLIP2.055226_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.055226
Chromosome:chromosome 12
Location:7498541
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g556750 TIC32,TIC32-1,SDR22 Short-chain dehydrogenase/reductase; (1 of 2) 1.1.1.330 - Very-long-chain 3-oxoacyl-CoA reductase / Very-long-chain beta-ketoacyl-CoA reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAATGATCTTACGTGGGACCTGGGGATCGCGAGGGCGGAAGGCGGGAG
Internal bar code:ATATTTTTCTCTTGATTTAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1030
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGATCGTCCATCGGCATG
Suggested primer 2:TTCGTACCGTGCAGTTCGAA