| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055239 |
| Chromosome: | chromosome 7 |
| Location: | 4325569 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341250 | (1 of 100) IPR001810 - F-box domain | CDS | |
| Cre07.g341251 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGGCTGCGCATCCTGCAACGAGTGGCGCCGCGGCTGCGATACGCTGCC |
| Internal bar code: | AATGACACAGAGTTTCGAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1939 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGTTGATGGTTGTGCAG |
| Suggested primer 2: | GAAGACACCACGAGTCAGCA |