| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.055283 |
| Chromosome: | chromosome 10 |
| Location: | 1898376 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431350 | TGL16 | (1 of 4) PTHR12406//PTHR12406:SF27 - CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED // SUBFAMILY NOT NAMED; Putative triacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGATCAACTGCGCCATGGCCAGCGCACATATCCCAATGCTGCTAGACC |
| Internal bar code: | AGGCCTTTGTTGCGCAGAGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1819 |
| LEAP-Seq percent confirming: | 83.6364 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGTACCCAACCTGCATG |
| Suggested primer 2: | TCTTGTCGACTGGGGTTTCG |