| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.055285 |
| Chromosome: | chromosome 12 |
| Location: | 4472902 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520350 | ALDH12A1,ALD1,ALDH12 | (1 of 1) 1.2.1.88 - L-glutamate gamma-semialdehyde dehydrogenase / Pyrroline-5-carboxylate dehydrogenase; Aldehyde dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATTAACGGGCCGGGGCAGCTGGCTTGCAATCCAAGCGTGGCTGCAGCA |
| Internal bar code: | CTAGTCTTTGTATATTAAAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 269 |
| LEAP-Seq percent confirming: | 12.1212 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAAAGTGCACCCACAGC |
| Suggested primer 2: | ACCAATTCCTTCCTCGGCTG |