Insertion junction: CLIP2.055296_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.055296
Chromosome:chromosome 13
Location:2303318
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g578800 LYR5 Complex 1 protein (LYR family); (1 of 3) IPR008011//IPR026868 - Complex 1 LYR protein // LYR motif-containing protein 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCCCAGCAAGAAGCGGACCTCCATCATCCGGGAAATCAAAGAAGAGTT
Internal bar code:CGGTTCTACAAATTTTTGTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:311
LEAP-Seq percent confirming:62.963
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTTGCACTTGAGCACAC
Suggested primer 2:GTCGGCCTTCACAACACAAC