| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055305 |
| Chromosome: | chromosome 3 |
| Location: | 3113061 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164500 | (1 of 1) IPR026571 - Transmembrane protein 186 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCCGATGTTTGCCGAAAATGATAGTAGCACAGCCGACGCCACCCACC |
| Internal bar code: | ACGTATGAAGCTTTTAGTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1146 |
| LEAP-Seq percent confirming: | 8.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCATGTGCCTCCTACAC |
| Suggested primer 2: | CAGTACGACGTGACCACACA |