Insertion junction: CLIP2.055316_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.055316
Chromosome:chromosome 14
Location:239844
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g609202 Conserved Protein With Tyrosinase Domain; (1 of 1) 3.4.24.7 - Interstitial collagenase / Vertebrate collagenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTAGGGGTGGAGGGTTGTAAATTCCAGCCCAAAGGGGGGGTGAGGGGT
Internal bar code:TAGTTAGTCATCTGTAGTTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:398
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCCCACAAACTACCCCC
Suggested primer 2:TGTGAGAAGCCGGGTCATTC