Insertion junction: CLIP2.055316_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.055316
Chromosome:chromosome 14
Location:239858
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g609202 Conserved Protein With Tyrosinase Domain; (1 of 1) 3.4.24.7 - Interstitial collagenase / Vertebrate collagenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCTCCACCCGCCCACTCAACACGGGCTGGCTGCAGAACGAGTTCGCG
Internal bar code:TTGAGTGTGGACTTCAGCATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:892
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGAGAAGCCGGGTCATTC
Suggested primer 2:CCTTCCCACAAACTACCCCC