| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.055326 |
| Chromosome: | chromosome 14 |
| Location: | 3731753 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631550 | (1 of 1) PTHR23130:SF67 - FERRIC-CHELATE REDUCTASE 1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAGAAGGTTGCACCCAAACGCACAGCAGGACATTGGGCCCGGCAGGGC |
| Internal bar code: | TTAAATGATTTCAATGTTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3738 |
| LEAP-Seq percent confirming: | 72.4638 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACACGATGTGATGTCGC |
| Suggested primer 2: | TATGTGAGCGCAGGAAAGCA |