| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.055334 |
| Chromosome: | chromosome 12 |
| Location: | 9055748 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544650 | (1 of 10) PF13489 - Methyltransferase domain (Methyltransf_23) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAGACAACAGCGCCTTCCTGCTAATTGCTATCGTACTCGCGCAGGGGC |
| Internal bar code: | GTCTATATATGGCCGGGGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2606 |
| LEAP-Seq percent confirming: | 35.8974 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACATCGGGTCCTCGAAAC |
| Suggested primer 2: | TTGCGTTGTGGTTGGAACAC |