Insertion junction: CLIP2.055341_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.055341
Chromosome:chromosome 6
Location:34030
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g248800 (1 of 1) PTHR31109:SF2 - PROTEIN FAM207A CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCACGGAAGGTGCAATTTCTGGACAGTAAGTTGAGAGGTCGCAGGGCA
Internal bar code:GGCTGCTAGTGGTTAGTTTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2680
LEAP-Seq percent confirming:53.3333
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGACTACGGTTTCCACGG
Suggested primer 2:GGGCGCGACATATCTACACA